What I learned from my bacteriology labs

There are three mains things I learned from my bacteriology labs:

1.  Growing pure cultures of bacteria is a pain.  It takes a long time and it can be very finicky.
2.  Growing bacterial cultures smell.  A lot.
3.  When trying to identify an unknown bacteria, even if you follow all the steps right, you can still get the answer wrong.

Two weeks ago we had a series of 4 bacteriology labs.  The theme running through the 4 labs was to identify unknown bacteria from a urine sample, mastitic milk and an abscess. We were given a "road map" of tests we could run and a clue that one sample had two bacteria, giving a total of 4 bacteria to identify.  After culturing the samples over night after the first lab, we started on to the tests on day two.  The first problem was sorting out which culture had two bacterial species.  After much confusion and discussion with the lovely lab assistant we discovered that the culture plate which was supposed to have two bacterial species was overrun with one bacteria, leaving one sole bacterial colony about the size of a pen tip of the second type.  After incredible luck (and my lab partners very steady hands) we were able to culture the two species onto two different plates for the next lab.

The next incident occurred when we ran a test called a microbat test.  Essentially you read off colours to be either positive or negative from a bunch of reaction wells.  You enter those results into a computer and voila!  The computer spits out the genus and species of your bacteria.  The lab assistant looked at our results and suggested we run the microbat again (so essentially telling us our results were wrong).  My partner had run the first one, so I ran the second one.  Guess what?  We got the same results!  When it came time to discuss our results with the professor, we had identified three of four species correctly.  We explained our dilemma with the microbat test and he told us "That's bacteriology for you!  Even if you follow all the steps and perform them correctly it doesn't always work."  There was a prize to be won if you got all your bacterial species correct.  Although we didn't get them all right, the professor took pity on us and gave us a prize anyways.

Not a bad prize split between two people!

Although I ultimately did enjoy the bacteriology labs, I don't think veterinary bacteriologist will be high on my career list.